Unlike London Underground most lines on the U-bahn run all night. We were out in Kreuzburg and had to get back to Rosenthaler Platz at 2.30am - think Elephant and Castle to Kings Cross. We went to the U-bahn at Mariennstrasse and found that trains were still running. We were able to get our connection OK too. Having said that, it's probably worth checking a timetable as some lines do stop at about 1am.
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