Instead of queueing for two hours to get in the Uffizi or one hour for the Accademia or Pitti Palace, call the hotline to book tickets for a three euro surcharge per person. They'll give you a booking number and you pay at the museum's ticket office. Number valid for all state musuems, hence the above plus San Marco, Bargello Archaeological Museum etc.
+39 055 294883
You should book before you go. We tried to book on our first day but the next available tickets were a week later so we missed out.